《植物生理学报》 2015, 51(7): 1055-1060

6-BA和硝酸银对三裂叶野葛毛状根生长及异黄酮含量的影响

何含杰^1,2, 施和平^2,*, 王震³

¹中南林业科技大学林业生物技术湖南省重点实验室/生物发育工程及新产品研发协同创新中心, 长沙410004; ²华南师范大学生命科学学院, 广东省植物发育生物工程重点实验室, 广州510631; ³上海现代哈森(商丘)药业有限公司, 河南商丘476000

通信作者：施和平；E-mail: shihp@scnu.edu.cn；Tel: 020-85214793

摘　要：

本文研究了6-苄氨基腺嘌呤(6-BA)和硝酸银(AgNO₃)组合对固体培养的三裂叶野葛毛状根生长、异黄酮化合物含量及抗氧化酶活性的影响。结果表明, 不同浓度的6-BA和30 μmol•L^-1 AgNO₃组合抑制三裂叶野葛毛状根的生长, 促进毛状根中异黄酮化合物的合成与积累, 但降低总异黄酮化合物和可溶性蛋白含量、超氧化物歧化酶和过氧化物酶活性水平, 促进细胞程序性死亡的发生。这说明6-BA和AgNO₃组合对三裂叶野葛毛状根的生长和异黄酮化合物的积累具有重要的调控作用。

关键词：三裂叶野葛; 毛状根; 6-BA; AgNO₃; 异黄酮化合物; 抗氧化酶活性; 细胞程序性死亡

收稿：2015-04-01   修定：2015-06-17

资助：湖南省自然科学基金项目(2015JJ3178)、长沙市科技攻关资金专项项目(K1406011-21)和中南林业科技大学青年基金项目(QJ2013003A)。

Effects of 6-Benzylaminopurine and Silver Nitrate on the Growth and Isoflavone Contents in Culture of Pueraria phaseoloides Hairy Roots

HE Han-Jie^1,2, SHI He-Ping^2,*, WANG Zhen³

¹Hunan Provincial Key Laboratory of Forestry Biotechnology/Cooperative Innovation Center of Engineering and New Products for Developmental Biology, Central South University of Forestry and Technology, Changsha 410004, China; ²Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China; ³Shanghai Hasen-Modern (Shangqiu) Pharmaceutical Company Limited, Shangqiu, Henan 476000, China

Corresponding author: SHI He-Ping; E-mail: shihp@scnu.edu.cn; Tel: 020-85214793

Abstract:

Using hairy roots of Pueraria phaseoloides cultured on solid media as an experiment material in this paper, the effects of 6-benzylaminopurine (6-BA) in combination with silver nitrate (AgNO₃) on its growth, isoflavone compound contents, and antioxidase activities were studied. The results showed that the growth of P. phaseoloides hairy roots, treated by different concentrations of 6-BA in combination with 30 μmol•L^-1 AgNO₃, was inhibited, and the synthesis and accumulation of isoflavone compounds in hairy roots per gram was enhanced compared with control. Meanwhile, the contents of total isoflavone compounds and soluble protein, and the activities of superoxide dismutase (SOD) and peroxidase (POD) in hairy roots were decreased, and the occurrence of programmed cell death was accelerated after treatment with different concentrations of 6-BA in combination with AgNO₃. These results indicated that 6-BA in combination with AgNO₃ had important regulation on growth and accumulation of isoflavone compounds in P. phaseoloides hairy roots.

Key words: Pueraria phaseoloides; hairy roots; 6-BA; AgNO3; isoflavone compounds; antioxidase activities; programmed cell death